Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase

In this study, we investigated the influence of different modes magnetic mixing on effective enzyme activity aspartate ammonia-lyase from Pseudomonas fluorescens immobilized onto epoxy-functionalized nanoparticles by covalent binding (AAL-MNP). The specific AAL-MNPs in traditional shake vial method was compared to MNP-based biocatalyst two devices designed for agitation. first device agitated moving permanent magnets at opposite sides a x-axis direction (being perpendicular y-axis vial); second unsettled MNP rotating around vial. vial, substrate and move same with pattern. agitation modes, MNPs responded differently external field magnets. axial mode, formed cloud inside whereas they ring. Especially, generated small fluid flow enabling reaction mixture, leading enhanced